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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Blood. 2011 Aug 5;118(13):10.1182/blood-2010-09-311076. doi: 10.1182/blood-2010-09-311076

Figure 1. Siglec-9 interacts with VAP-1.

Figure 1

(A) The amino acid sequence of the enriched phage clone, the corresponding region of Siglec-9 and the adhesion of the phage peptide to recombinant VAP-1 (rVAP-1 100 ng/well) measured using EIA. The results are mean ± SEM from three separate experiments and triplicate wells in each experiment. (B) Binding of the Siglec-9 peptide (Sig-9 pept marked red in A) to CHO-VAP-1 transfectants and CHO-mock controls. The results are presented as relative fluorescence intensity and are mean ± SEM from two separate experiments each having triplicate wells. (C) Binding of CFSE labeled Siglec-9 transfectants to CHO cells expressing VAP-1 and mock controls. The results are mean ± SEM of fluorescent intensities measured by fluorometer from seven separate experiments each having duplicate wells. **P < 0.01; ***P < 0.001.