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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Mol Biochem Parasitol. 2005 Sep 6;144(2):10.1016/j.molbiopara.2005.08.009. doi: 10.1016/j.molbiopara.2005.08.009

Fig 1.

Fig 1

Expression of T7RNAP from a Tet-inducible RRNA promoter. (A) NotI-digestion targets pRPT7pol to an RRNA locus. BLA, blasticidin resistance. Arrows represent promoters. RNA processing regions and promoters: ALD, aldolase; PRO, procyclin; TUB, tubulin. (B and C) Western blot analysis of T7RNAP expression. The blots were probed with primary mouse α-T7RNAP (1:5000). The lower panels show coomassie-stained gels as loading controls. (B) Six independent cell-lines expressing TetR and transfected with pRPT7pol express different levels of T7 polymerase upon induction with 1μg ml−1 Tet. Lane 1: un-induced cell line 1. (C) Induction of cell line 1 with 1ng to 1μg ml−1 Tet showed that T7 polymerase expression was dose dependent, peaking in the presence of 50ng ml−1 Tet. (D) Growth analysis was carried out in triplicate and populations were diluted as shown. Error bars: standard deviation.