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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2013 Oct 2;191(10):10.4049/jimmunol.1301490. doi: 10.4049/jimmunol.1301490

Figure 1. NLRP3 dependent cell membrane damage.

Figure 1

(A)LPS primed or unprimed WT and NLRP3−/− BMDM were treated with 20 µM nigericin or 3 mM ATP for thirty minutes with or without KCl. LDH release was recorded as the percent released into the supernatant by five minutes of Triton treatment. LDH assay was allowed to develop for two hours and readings were taken as an average of two wells. Error bars represent mean ± s.d. (n=3, *, P<0.01; LPS Nig WT vs KO: P=0.009; WT LPS Nig vs WT LPS Nig KCl: P=0.004). (B) Conversion of MTT reagent added to cell lysates was measured as absorbance of the colored product formazan. Data were acquired as the percent of absorbance of each well/absorbance of Triton-X treated control cells and graphed as MTT reagent remaining unconverted by mitochondrial reductases. Readings were taken as an average of two wells. Error bars represent mean ± s.d. (n=4, ***, P<0.0001; n=3, **, P=0.0155).