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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: J Immunol. 2013 Oct 2;191(10):10.4049/jimmunol.1301490. doi: 10.4049/jimmunol.1301490

Figure 2. Loss of cellular ATP and decrease in OCR are NLRP3 independent.

Figure 2

WT or NLRP3 −/− BMDM were LPS primed and (A) treated with nigericin for thirty minutes with or without KCl. Supernatants were collected and ATP levels measured. Error bars represent ± s.d. (n≥6, *, P=0.0103; ns, p=0.7941) WT BMDM (B) or NLRP3−/− BMDM (C) were treated with nigericin immediately before inserting plate in Seahorse machine. OCR was recorded as an average of 3–4 wells. Media (line a), oligomycin (b), FCCP (c), and rotenone (d) were added as indicated. Error bars represent ± s.d.(n=4, at 45 min, ns, P=0.3099).