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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Int J Cancer. 2011 Mar 29;129(12):10.1002/ijc.25946. doi: 10.1002/ijc.25946

Fig. 1.

Fig. 1

Effects of gal-3 silencing on PanC-1, AsPC-1 and BxPc-3 cell proliferation. a Gal-3 expression in human pancreatic cancer cell lines. Human pancreatic cancer cells were transfected with either gal-3 siRNA or control-siRNA, or were left untreated. Total cell lysates were isolated after 48 h of transfection and gal-3 expression levels were detected by western blotting using an anti-human gal-3 antibody. β-actin was also used as a loading control. Densitometry (mean ± SD) revealed over 80% knockdown of gal-3 in gal-3-siRNA-transfected cells (n = 3). *P < 0.05 versus control-siRNA. b Proliferation assays. The mean ± SD absorbance (n = 3) levels of the pancreatic cancer cells are shown. PanC-1, AsPC-1, and BxPc-3 cells were separately cultured in 96-well microplates, the indicated reagent (untreated, control-siRNA, or Gal-3-siRNA) was injected onto cells after 0, 24, 48, or 72 h of culture, and the plates were incubated for an additional 2 h. The absorbance levels were detected using a microplate reader.