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Comparison of Gel Analysis Assay for RT-PCR Success to Sequence Analysis Assay
| Sequence analysis | One right size band | Multiple bands ≥ right size | No right size band | Total |
|---|---|---|---|---|
| Hit | 65 | 35 | 54 | 154 |
| Non-hit | 5 | 38 | 166 | 209 |
| Total | 70 | 73 | 220 | 363 |
Products that gave high-quality sequence with a clear spliced alignment to the genome in the target region are classified as hits; all others are classified as non-hits.
