Figure 1.

Constitutive secretion of VEGF by human uveal melanoma cells and normal uveal melanocytes. Three different primary cultures of uveal melanocytes (UM1-3) and uveal melanoma cells from 3 different cell lines (SP6.5, M17, and C918) were plated into 24-well plates. After 24 h, culture medium was removed and cultures were washed by PBS. Serum-free culture medium was added and cultured for 24 h. Conditioned media were collected, and the amount of VEGF protein was determined by using the human VEGF Quantikine ELISA kit. VEGF levels in conditioned culture medium were expressed as pg/mL. VEGF levels in culture medium from melanoma cells were significantly higher than that from normal melanocytes (P < 0.05). Data are mean ± SD (n = 3).