Table 3.

Comparison between the dilutions of the vaccine strains (for MG and MS) and the Ct values obtained in three replicates.

	Concentration	Multiplex PCR		Specific PCR
		MS Ct# (DP*)	MG Ct# (DP*)	MS Ct# (DP*)	MG Ct# (DP*)
MS	High	22.0 (0.1)	Negative	21.6 (0.2)	Negative
	Intermediate	27.0 (0.4)	Negative	26.5 (0.4)	Negative
	Low	35.4 (0.8)	Negative	37.1 (0.8)	Negative
MG	High	Negative	22.0 (0.1)	Negative	22.1 (0.1)
	Intermediate	Negative	26.5 (0.3)	Negative	26.7 (0.3)
	Low	Negative	32.0 (0.5)	Negative	31.5 (0.3)

^#Average Ct of the three replicates.

^*Standard deviation.