Figure 1.

Nutlin-3 treatment induced upregulation of p53, p21, MDM2 and DR5 (membrane) expression. (A) Treatment of A2780, H460 and Lovo with increasing concentrations of nutlin-3 for 24 h resulted in a dose-dependent upregulation of p53, MDM2, p21 and DR5 protein levels as determined by western blotting. (B) DR4, DR5 and DcR2 membrane expression levels were measured using FACS analysis demonstrating that nutlin-3 enhanced DR5 levels dose-dependently, whereas DcR2 remained unchanged. In H460 and Lovo, DR4 levels decreased upon treatment. DcR1 was not detected. Presented data are representative for at least three independent experiments and mean MFI levels are shown ±s.d. *P<0.05, **P<0.01 compared with DR5 MFI at 0 μM nutlin-3. ^#P<0.05 compared with DcR2 MFI at 0 μM nutlin-3.