Figure 5.

Western blot analyses for Caspase 2, Caspase 3, and PARP-1 in the four drug-sensitive human osteosarcoma cell lines after treatment with VX-680 and ZM447439. Lines with U-2OS+CDDP5 μg and Saos-2+CDDP10 μg were used as positive controls to detect Caspase 3 and PARP-1 cleavage induced by the treatment for 48 h with CDDP 5 μg ml⁻¹ or CDDP 10 μg ml⁻¹, respectively. CTR, control non-treated cells cultured in drug-free and DMSO-free medium; DMSO, control non-treated cells cultured in the presence of DMSO concentrations corresponding to those of drug-treated samples; VX-680 or ZM447439, cells treated with IC50 or two-fold IC50 (VX-680 × 2 or ZM447439 × 2) dosage of each Aurora kinase inhibitor drug.