Table 1. Gene and protein knockdown obtained with siRNA transfection in three human OS cell lines.

Sample	%AURK-A mRNA	%AURK-A protein	Sample	%AURK-B mRNA	%AURK-B protein
U-2OS
NT-CTRa 24 h	100	100	NT-CTR 24 h	100	100
SCRb	104	124	SCR	130	131
siRNA AURK-A	11	0	siRNA AURK-B	22	10
NT-CTR 48 h	100	100	NT-CTR 48 h	100	100
SCR	112	119	SCR	105	131
siRNA AURK-A	14	14	siRNA AURK-B	17	2
NT-CTR 72 h	100	100	NT-CTR 72 h	100	100
SCR	115	106	SCR	113	108
siRNA AURK-A	17	15	siRNA AURK-B	28	24
Saos-2
NT-CTR 48h	100	100	NT-CTR 48 h	100	100
SCR	124	86	SCR	135	83
siRNA AURK-A	12	17	siRNA AURK-B	20	3
NT-CTR 72 h	100	100	NT-CTR 72 h	100	100
SCR	78	122	SCR	99	118
siRNA AURK-A	15	36	siRNA AURK-B	18	20
NT-CTR 96 h	100	100	NT-CTR 96 h	100	100
SCR	105	108	SCR	106	116
siRNA AURK-A	8	17	siRNA AURK-B	22	0
IOR/OS18
NT-CTR 48 h	100	100	NT-CTR 48 h	100	100
SCR	148	120	SCR	121	95
siRNA AURK-A	70	49	siRNA AURK-B	60	12
NT-CTR 72 h	100	100	NT-CTR 72 h	100	100
SCR	115	136	SCR	99	138
siRNA AURK-A	70	51	siRNA AURK-B	64	14
NT-CTR 96 h	100	100	NT-CTR 96 h	100	100
SCR	100	73	SCR	103	92
siRNA AURK-A	58	31	siRNA AURK-B	70	18

Abbreviations: AURK-A=Aurora kinase-A; AURK-B=Aurora kinase-B; OS=osteosarcoma.

^aNT-CTR, non-treated cells (controls).

^bSCR, cells transfected with scrambled siRNAs.