Table 1. Gene and protein knockdown obtained with siRNA transfection in three human OS cell lines.
Sample | %AURK-A mRNA | %AURK-A protein | Sample | %AURK-B mRNA | %AURK-B protein |
---|---|---|---|---|---|
U-2OS | |||||
NT-CTRa 24 h | 100 | 100 | NT-CTR 24 h | 100 | 100 |
SCRb | 104 | 124 | SCR | 130 | 131 |
siRNA AURK-A | 11 | 0 | siRNA AURK-B | 22 | 10 |
NT-CTR 48 h | 100 | 100 | NT-CTR 48 h | 100 | 100 |
SCR | 112 | 119 | SCR | 105 | 131 |
siRNA AURK-A | 14 | 14 | siRNA AURK-B | 17 | 2 |
NT-CTR 72 h | 100 | 100 | NT-CTR 72 h | 100 | 100 |
SCR | 115 | 106 | SCR | 113 | 108 |
siRNA AURK-A |
17 |
15 |
siRNA AURK-B |
28 |
24 |
Saos-2 | |||||
NT-CTR 48h | 100 | 100 | NT-CTR 48 h | 100 | 100 |
SCR | 124 | 86 | SCR | 135 | 83 |
siRNA AURK-A | 12 | 17 | siRNA AURK-B | 20 | 3 |
NT-CTR 72 h | 100 | 100 | NT-CTR 72 h | 100 | 100 |
SCR | 78 | 122 | SCR | 99 | 118 |
siRNA AURK-A | 15 | 36 | siRNA AURK-B | 18 | 20 |
NT-CTR 96 h | 100 | 100 | NT-CTR 96 h | 100 | 100 |
SCR | 105 | 108 | SCR | 106 | 116 |
siRNA AURK-A |
8 |
17 |
siRNA AURK-B |
22 |
0 |
IOR/OS18 | |||||
NT-CTR 48 h | 100 | 100 | NT-CTR 48 h | 100 | 100 |
SCR | 148 | 120 | SCR | 121 | 95 |
siRNA AURK-A | 70 | 49 | siRNA AURK-B | 60 | 12 |
NT-CTR 72 h | 100 | 100 | NT-CTR 72 h | 100 | 100 |
SCR | 115 | 136 | SCR | 99 | 138 |
siRNA AURK-A | 70 | 51 | siRNA AURK-B | 64 | 14 |
NT-CTR 96 h | 100 | 100 | NT-CTR 96 h | 100 | 100 |
SCR | 100 | 73 | SCR | 103 | 92 |
siRNA AURK-A | 58 | 31 | siRNA AURK-B | 70 | 18 |
Abbreviations: AURK-A=Aurora kinase-A; AURK-B=Aurora kinase-B; OS=osteosarcoma.
NT-CTR, non-treated cells (controls).
SCR, cells transfected with scrambled siRNAs.