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. 2013 Oct 29;109(10):2629–2635. doi: 10.1038/bjc.2013.645

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Copyright © 2013 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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The intratumoral FOXP3⁺ Treg are more suppressive than circulating FOXP3⁺ Treg. (A) Suppression of autogolous RC proliferation by CD25⁺CD4⁺ T cells isolated from TIL or PBL (from two patients). CFSE-labelled CD4⁺CD25⁻ T cells were co-cultured with unlabelled autologous CD4⁺CD25⁺ T cells isolated from TIL or PBL as indicated. After 4 days of stimulation with anti-CD3 antibody, CFSE⁺ CD4⁺ T cells were analysed for their proliferation by CFSE dilution. (B) Percentage of suppression of TIL-Treg was compared with that of PBL-Treg from two patients, respectively.