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. 2013 Nov;30(11):697–705. doi: 10.1089/ees.2013.0067

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 2. — 16S rRNA gene neighbor-joining tree showing relationship of representative sequences from SIP-derived OTUs in this study (in bold) to nearest relatives. The total number of clones represented by each sequence is indicated in brackets. GenBank accession numbers are displayed in parentheses. A sequence representing AG1 from SIP of the untreated soil is underlined. The tree was bootstrapped 1,000 times and circles at nodes indicate either ≥95% (open circles) or ≥50% (closed circles) bootstrap support. The 16S rRNA gene sequence of Mycobacterium vanbaalenii was used as an outgroup. OTUs, operational taxonomic units; SIP, stable-isotope probing.