Table 1.
Quantitative Real-Time Polymerase Chain Reaction Primers Used in This Study
| Target | Primer name | Sequence (5′→3′) | Amplicon size (bp) | Annealing temp (°C) | Amp. eff.a | No. seqs.b | Ref. |
|---|---|---|---|---|---|---|---|
| OTU-6 | ANT-6F | AAC AAC TGA GGG AAA CTT CA | 113 | 57.5 | 1.70 | 1172 | This study |
| ANT-6R | TTG GTG AGC CAT TAC CTC | ||||||
| OTU-11 | ANT-11F | CGG ATT ACA GAG ATG TTT TC | 149 | 56 | 2.19 | 0 | This study |
| ANT-11R | TAG AGT TCC CAA CTG AAT GA | ||||||
| OTU-12 | ANT-12F | AAA TCC CAG AGC TCA ACT CT | 75 | 58 | 2.09 | 5 | This study |
| ANT-12R | CGC AGT TCC ACT TAC CTC TT | ||||||
| OTU-19 | ANT-19F | ACA TCC CGG TCG CGG ACT | 63 | 60 | 1.85 | 1782 | This study |
| ANT-19R | ACC YGT CTC CGG TCC AG | ||||||
| OTU-41 | PG2.4F | CCA AGC CGA CGA CGG GTA G | 94 | 59 | 1.99 | 742 | (Jones et al., 2011a) |
| PG2.4R | TTC CCC ACT GCT GCC TC |
a
Amplification efficiency.
b
Number of sequences in the RDP-II database (release 10, update 29; June 1, 2012) that match both primers with no mismatches as determined by the ProbeMatch function (Cole et al., 2009).
OTU, operational taxonomic unit.