Table 1.
Total Phenolic and Proanthocyanidin Content in Pinus brutia Bark Extracts
Total phenolic content (mg gallic acid/g extract) | Proanthocyanidin content (mg (+)-catechin/g extract) | |
---|---|---|
PbE | 412.42 ± 7.56††††,‡‡‡‡,####,$ | 225.79 ± 3.94††††,$$$$ |
PbE1 | 366.71 ± 5.63****,‡‡‡‡,####,$$$ | 10.05 ± 0.22****,‡‡‡‡,####,$$$$ |
PbE2 | 448.90 ± 1.39****,††††,####,$$$$ | 231.41 ± 1.24††††,$$$$ |
PbE3 | 303.79 ± 7.34****,††††,‡‡‡‡,$$$$ | 229.10 ± 1.13††††,$$$$ |
PbE4 | 393.49 ± 5.39*,†††,‡‡‡‡,#### | 250.40 ± 1.44****,††††,‡‡‡‡,#### |
Significant differences between extracts are indicated: *P < .05, ****P < .001 vs. PbE; †††P < .002, ††††P < .001 vs. PbE1; ‡‡‡‡P < .001 vs. PbE2; ####P < .001 vs. PbE3; $P < .05, $$$P < .002, $$$$P < .001 vs PbE4.
PbE, crude extract of P. brutia bark with 80% (v/v) methanol (18.36 g); PbE1, PbE fractionated with diethyl ether (2.18 g); PbE2, PbE fractionated with ethyl acetate (1.84 g); PbE3, PbE fractionated with n-butanol (8.59 g); PbE4, remaining aqueous phase (5.37 g).