Table 3.
Trolox equivalent antioxidant capacity assay TEAC | Reducing power assay EC50 (μg/mL) | Superoxide anion radical scavenging assay EC50 (μg/mL) | Hydroxyl radical scavenging assay EC50 (mg/mL)a | 15-LO inhibition assay EC50 (μg/mL) | |
---|---|---|---|---|---|
PbE | 1.47 ± 0.02††††,‡,####,$$$$,¶¶¶¶ | 9.17 ± 0.13††††,‡,####,$,¶¶¶¶ | 39.37 ± 0.85††††,‡‡‡,####,$$$,¶¶¶¶ | 0.5 ± 0.0†††† | 22.47 ± 0.75††††,‡,##,¶¶ |
PbE1 | 1.08 ± 0.04****,‡‡‡‡,####,$$$$,¶¶¶¶ | 10.27 ± 0.11****,‡‡‡‡,###,$$,¶¶¶¶ | 70.30 ± 2.65****,‡‡‡‡,###,$$$$,¶¶¶¶ | 1.07 ± 0.06****,‡‡‡‡,####,$$$$,¶¶¶¶ | 34.43 ± 2.25****,‡‡‡,###,$$$$,¶¶¶¶ |
PbE2 | 1.43 ± 0.01*,††††,####,$$$$,¶¶¶¶ | 9.49 ± 0.01*,††††,####,¶¶¶¶ | 51.10 ± 2.66***,††††,$$$$,¶¶¶¶ | 0.53 ± 0.06††††,¶¶¶¶ | 24.3 ± 0.4*,†††,$,¶¶¶¶ |
PbE3 | 0.89 ± 0.01****,††††,‡‡‡‡,$$$$,¶¶¶¶ | 11.38 ± 0.24****,†††,‡‡‡‡,$$$$,¶¶¶¶ | 54.93 ± 2.85****,†††,$$$$,¶¶¶¶ | 0.6 ± 0.0††††,$ | 24.9 ± 0.5**,†††,$$$$,¶¶¶¶ |
PbE4 | 1.54 ± 0.00****,††††,‡‡‡‡,####,¶¶¶¶ | 9.54 ± 0.18*,††,####,¶¶¶¶ | 33.5 ± 1.1***,††††,‡‡‡‡,####,¶¶¶¶ | 0.47 ± 0.06††††,#,¶¶¶¶ | 22.93 ± 0.55††††,‡,##,¶¶¶ |
Quercetin | 5.62 ± 0.04****,††††,‡‡‡‡,####,$$$$ | 3.4 ± 0.2****,††††,‡‡‡‡,####,$$$$ | 26.63 ± 0.75****,††††,‡‡‡‡,####,$$$$ | 0.2 ± 0.0††††,‡‡‡‡,$$$$ | 18.70 ± 0.85**,††††,‡‡‡‡,####,$$$ |
For the hydroxyl radical scavenging assay, statistical comparisons for PbE vs. PbE3, PbE vs, quercetin, and PbE3 vs. quercetin were not determined.
Significant differences between samples (extracts, quercetin) are indicated: *P < .05, **P < .01, ***P < .002, ****P < .001 vs. PbE; ††P < .01, †††P < .002, ††††P < .001 vs. PbE1; ‡P < .05, ‡‡‡P < .002, ‡‡‡‡P < .001 vs. PbE2; #P < .05, ##P < .01, ###P < .002, ####P < .001 vs. PbE3; $P < .05, $$P < .01, $$$P < .002, $$$$P < .001 vs. PbE4; ¶¶P < .01, ¶¶¶P < .002, ¶¶¶¶P < .001 vs. quercetin.