FIG. 4.

Yield and purity of K18+ simple epithelial cells as a function of day 0 cell density. (A) Purity of H9-derived simple epithelial cells at day 7 of differentiation at various starting densities as measured by flow cytometry. (B) Yield of simple epithelial cells at day 7 of differentiation calculated by normalizing the absolute number of K18+ epithelial cells at day 7 to the starting number of Nanog+ cells at day 0. Error bars indicate standard deviation of three independent replicates. Color images available online at www.liebertpub.com/tec