FIG. 5.

Epithelial commitment of hPSCs on defined substrates. (A) Method of generating K18+ simple epithelial cells by culturing hPSCs on a StemAdhere or Synthemax substrate in lieu of a Matrigel substrate. (B) Quantification of flow cytometry data exhibiting highly enriched K18+ or p63+ cell populations generated on Matrigel, StemAdhere, and Synthemax substrates. Error bars indicate standard deviation of three independent replicates for each condition (*, ^# indicates p>0.05 compared to Matrigel condition). (C) Representative flow cytometry dot plots showing homogenous K18+ or (D) p63+ populations generated from H9 hESCs cultured on defined substrates compared to Matrigel on day 7 of differentiation. Isotype controls are shown for each condition. (E) Immunofluorescence images of K18 expression in H9 hESC-derived cell populations at day 7 of differentiation. Scale bar in all panels is 100 μm. Color images available online at www.liebertpub.com/tec