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. 2013 Jun 4;19(12):949–960. doi: 10.1089/ten.tec.2013.0011

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Copyright 2013, Mary Ann Liebert, Inc.

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FIG. 8. — Stratification and terminal differentiation of H9 hESC-derived keratinocyte progenitors to epidermal keratinocytes. (A) H&E stain of a cross-section of H9 hESC-derived K14+/p63+ cell populations generated using the defined epithelial differentiation system on Synthemax substrates after culture for in an organotypic culture system. Inset shows multiple cell layers. Immunohistochemical stains were performed on cross-sections of these tissues showing the presence of (B) K14 (red), (C) p63 (red), and terminal-differentiation markers (D) filaggrin (green), (E) involucrin (green), and (F) K10 (green). Scale bar in all panels is 50 μm.