Fig. 4.

Immunostaining detects Kif20b protein in the central spindle and midbody of normal but not magoo mutant MEFs. Fibroblasts cultured from E12.5 magoo mutant and control littermate mouse embryos were immunostained for Kif20b (red) and aurora B kinase (AurB, green). In control anaphase cells, aurora B localizes to microtubules of the central spindle, and Kif20b is barely seen as a few small puncta. In early cytokinesis, Kif20b protein is detected on the compacting central spindle of control but not mutant cells. In late cytokinesis, Kif20b appears concentrated at the inner and outer flanks of the midbody in control cells but is not detectable in mutant midbodies. Scale bar: 15 μm.