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Disruption of the anterior-posterior boundary in the secondary palate in Pax9del/del mutant embryos. (A,B) Direct comparison of Shh (left half) and Shox2 (right half) mRNA expression patterns in the palatal shelf pairs in wild-type (A) and Pax9del/del mutant (B) embryos at E13.5. (C,D) Dual color in situ hybridization detection of Shh (brown) and Barx1 (turquoise) mRNAs in the palatal shelves of E13.5 wild-type (C) and Pax9del/del mutant (D) embryos.
