Fig. 3.

Br upregulation coincides with high ecdysone biosynthetic enzyme levels and the ecdysone peak. PGs from wild-type (w¹¹¹⁸) larvae, at the indicated times AEL, were dissected and immunostained for Phm and Dib (red) and Br (green). DAPI (cyan) staining is shown for samples stained for Dib. Dotted lines encircle PG cells. All samples were processed and examined using the same microscope settings. Representative images from the PG for each experimental group (n≥5) are shown. Scale bars: 25 μm.