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. 2013 Dec 1;140(23):4788–4796. doi: 10.1242/dev.094490

Fig. 4.

Fig. 4.

DAC-2-25 targets ectodermal epithelial cells in a cell-autonomous manner. (A) A schematic representation of the method used to produce chimeric Hydra (see Materials and methods for more details). (B) The chimera described in A was exposed to DAC-2-25 and ectopic tentacles arose in a manner similar to that seen in AEP animals. (C) A chimera with Zürich ectoderm and AEP endoderm did not respond to DAC-2-25. (D,E) Ectopic tentacles did not form in an animal with AEP i-cells and Zürich epithelial layers. (F,G) Mosaic Hydra were produced when chimeras (shown in D,E) budded. These mosaics were exposed to DAC-2-25 and ectopic tentacles were seen only in epithelial tissue derived from the AEP donor.