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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Mol Imaging Biol. 2013 Jun;15(3):10.1007/s11307-012-0596-5. doi: 10.1007/s11307-012-0596-5

Table 2.

Primers used in this study

Primer sequence Description Source
Fwd: TCGACCAAGCTTTCGGTACCAGCCCGGGAGAGCTCAAGGATCCAAG
Rev: AATTCTTGGATCCTTGAGCTCTCCCGGGCTGGTACCGAAAGCTTGG		 Multiple Cloning site for pENTR4D: SalI - HindIII - KpnI - SmaI - SacI - BamHI - EcoRI. Gil 2011[16]
Fwd: TCGACCAAGCTTGCCACCATGCCCACGCTACTGCGGGT
Rev: CTAGATATCTCGAGTTATCAGTTAGCCTCCCCCATCT		 sr39TK from pMSCV-sr39TK-IRES-YFP. HindIII/XhoI directional cloning. This study
Fwd: TCGACCAAGCTTGCCACCATGTCAGTGATCTGTGTCGA
Rev: TCTAGATATCTCGAGTTACTATGGGCAATGCTTCCGAT		 TK2 and TK2-N93D/L109F from pMSCV-TK2 and pMSCV-TK2-N93D/L109F respectively. HindIII/XhoI directional cloning. This study
Fwd: AGTCGACCAAGCTTGCCACCATGGCTACTCCTCCTAAACG
Rev: TGGGTCTAGATATCTCGAGTTATCAAAGTGTTGACAGAAAT TCC		 dCK-WT, dCK-DM, and dCK-TM from pMSCV-hudCK-triple mutant-IRES-YFP. HindIII/XhoI directional cloning. This study
Fwd: TAAAGCTTAGCGACGATGGCCTCCAAG
Rev: TTCTCGAGCTACTGTTCGTTCTTCAGC		 RLuc8.6-535 from pRV2011. HindIII/XhoI directional cloning. This study
Fwd: ACTTAAGCTTGCCACCATGGAAGATGCCAAAAACATT
Rev: AACTCGAGAATTATTACACGGCGATCTTGC		 Luc2 from pGL4.13. HindIII/XhoI directional cloning. This study
Fwd: ACTTAAGCTTGCCACCATGGCTTCCAAGGTGTACGAC
Rev: AACTCGAGAATTATTACTGCTCGTTCTTCAGCAC		 hRLuc from phRL-TK. HindIII/XhoI directional cloning. This study
Fwd: CAAGGGTTGAGTACTTGTTTAGGGTTA
Rev: GGTGGGTAGAGAGAAGAAATATCTGACT
Probe: TAMRA-AGGACAATGGCCTTGGCTGGACAA-BHQ2A		 TaqMan qPCR primer against mouse Oct4 promoter region Gil 2011 [16]
Fwd: TTG TGGTTCTTGCAGATATGGC
Rev: TCGGAATCCCGGCACC
Probe: FAM-CTCACCTGCCGCCTCCGTTTCC-TAMRA		 Taqman qPCR primer against adenovirus type 5 pX gene Gallaher 2009 [19]