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. Author manuscript; available in PMC: 2014 Sep 6.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Aug 7;438(4):10.1016/j.bbrc.2013.07.125. doi: 10.1016/j.bbrc.2013.07.125

Fig. 3.

Fig. 3

Regulation of fetuin-A mediated chemo-attraction by plasminogen and the dual abilities of fetuin-A to attract and promote invasion of tumor cells through Matrigel. Panel A, the chemo-attraction assays were done essentially as described in Section 2. The BT-549 cells (25,000 cells/well) were added to the upper wells of Boyden chemotaxis wells in 500 μl of SFM without or with the indicated concentrations of plasminogen (PL) and placed over lower wells containing either 500 μl of CM or fetuin-A (1 mg/ml) respectively. Panel B, the lower wells of Boyden invasion chambers contained either 500 μl of SFM, CM, or purified fetuin-A (1 mg/ml) in SFM. The BT-549 cells (25,000 cells/well in 500 μl of SFM) were added to the upper wells containing Matrigel. The error bars in A and B represent the means of three separate experiments under the same conditions ± SD (one way ANOVA *P < 0.001).