. Author manuscript; available in PMC: 2014 Oct 4.

Published in final edited form as: J Org Chem. 2013 Sep 25;78(19):10.1021/jo4015936. doi: 10.1021/jo4015936

Table 2.

ΔT_m values of duplexes between ONs modified with pyrene-functionalized adenine monomers and complementary DNA, measured relative to unmodified duplexes.^a

ON	Duplex	ΔT_m /°C
ON	Duplex	B =	K^b	L^b	M	R
B6	5′-GTG BTA TGC		+5.0	+11.0	+6.0	+4.5
D2	3′-CAC TAT ACG		+5.0	+11.0	+6.0	+4.5

B7	5′-GTG ATB TGC		+7.0	+14.0	+7.5	+8.5
D2	3′-CAC TAT ACG		+7.0	+14.0	+7.5	+8.5

D1	5′-GTG ATA TGC		+6.5	+11.5	+7.5	+8.5
B8	3′-CAC TBT ACG		+6.5	+11.5	+7.5	+8.5

D1	5′-GTG ATA TGC		+5.5	+12.0	+6.0	+6.5
B9	3′-CAC TAT BCG		+5.5	+12.0	+6.0	+6.5

ΔT_m = change in T_m values relative to unmodified reference duplex D1:D2 (T_m ≡ 29.5 °C); see Table 1 for experimental conditions; see Figure 1 for structures of monomers.

reference 39