. Author manuscript; available in PMC: 2014 Oct 4.

Published in final edited form as: J Org Chem. 2013 Sep 25;78(19):10.1021/jo4015936. doi: 10.1021/jo4015936

Table 4.

ΔT_m and DA values for DNA duplexes with interstrand zipper arrangements of pyrene-functionalized adenine monomers.^a

ON	Zipper	Duplex	ΔT_m[DA]/°C
ON	Zipper	Duplex	B =	K	L	M	R
B6	+3	5′-GTG BTA TGC		+12.0 [+1.5]	+13.0 [-10.0]	+16.0 [+4.0]	+10.0 [-1.0]
B9	+3	3′-CAC TAT BCG		+12.0 [+1.5]	+13.0 [-10.0]	+16.0 [+4.0]	+10.0 [-1.0]

B6	+1	5′-GTG BTA TGC		-7.0 [-18.5]	-8.0 [-30.5]	-7.5 [-21.0]	-7.5 [-20.5]
B8	+1	3′-CAC TBT ACG		-7.0 [-18.5]	-8.0 [-30.5]	-7.5 [-21.0]	-7.5 [-20.5]

B7	+1	5′-GTG ATB TGC		-5.0 [-17.5]	-7.0 [-33.0]	-8.0 [-21.5]	-7.0 [-22.0]
B9	+1	3′-CAC TAT BCG		-5.0 [-17.5]	-7.0 [-33.0]	-8.0 [-21.5]	-7.0 [-22.0]

B7	-1	5′-GTG ATB TGC		+14.0 [+0.5]	+23.5 [-2.0]	+22.0 [+7.0]	+15.0 [-2.0]
B8	-1	3′-CAC TBT ACG		+14.0 [+0.5]	+23.5 [-2.0]	+22.0 [+7.0]	+15.0 [-2.0]

ΔT_m = change in T_m values relative to unmodified reference duplex D1:D2 (T_m ≡ 29.5 °C); see Table 1 for experimental conditions.