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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: Science. 2008 Jul 17;321(5891):10.1126/science.1156970. doi: 10.1126/science.1156970

Fig. 1.

Fig. 1

GSNO facilitates the oligomerization of NPR1. (A) SA induces NPR1 monomer release as well as oligomerization. Nonreducing (–DTT) and reducing (+DTT) immuno-blot analysis revealed oligomeric (O), monomeric (M), and total (T) NPR1. (B) Re-oligomerization of monomeric NPR1-GFP in a cell-free assay. (C) NPR1 monomer disappears with an increasing concentra tion of GSNO but not of SNP or H2O2, while total protein levels are unaffected. (D) SA induces nuclear localization of the NPR1-GFP monomer in the wild type but not in the atgsnor1-3 mutant. (E) SA-induced PR-1 gene expression is compromised in atgsnor1-3 plants. PR-1 expression was determined by real-time polymerase chain reaction (PCR) and normalized with ubiquitin (UBQ). Error bars represent SD.