Analysis of VH and VL gene usage and CDR sequence of 1E4. Panel A, analysis of 1E4 VH and VL gene usage by IMGT/V-QUEST. a: identity to germline genes. Panel B, comparison of amino acid sequence of 1E4 VH gene with the anti-Neisseria meningitidis group B polysaccharide mAb SEAM12 and murine germline family IGHV13-2 by ClustalW program. A star (*) indicates the same amino acid residue identity in a position, while a dot (:) indicates a different residue, and a dash (-) indicates inserted spaces placed in the sequence to provide maximum identity. Panel C, comparison of the HCDR3 conjunction between 1E4 and SEAM12. N: nucleotide addition mutation; P: P deletion mutation.