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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: J Immunol. 2012 Oct 10;189(10):10.4049/jimmunol.1201622. doi: 10.4049/jimmunol.1201622

Figure 4.

Figure 4

Identification of 1E4-specific phage clones. Panel A, amino acid sequence alignment of nineteen 1E4 recognized phagotopes. The consensus residues are shadowed. Panel B, the reactivity of recombinant phage clones with 1E4 was analyzed by ELISA. A 96-well microtiter plate was coated with individual 1010 of purified phage particles and incubated with 5μg/ml of 1E4 with or without 2μg/ml of PI antigen. The Inhibition of 1E4 binding by PI antigen was measured by the inhibition index {(A490 without inhibitor-A490 with inhibitor)/A490 without inhibitor}.