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. Author manuscript; available in PMC: 2013 Nov 19.
Published in final edited form as: J Immunol. 2012 Oct 10;189(10):10.4049/jimmunol.1201622. doi: 10.4049/jimmunol.1201622

Figure 5.

Figure 5

Evaluation of the binding ability of the synthetic mimetic peptide m1E41920 and m1E41920-KLH conjugate with 1E4 by ELISA and competitive ELISA. Panel A, binding activity of m1E41920 and m1E41920-KLH conjugate with 1E4 was measured by ELISA. A 96-well microtiter plate was coated with m1E41920, m1E41920-KLH, control peptide, control-KLH or KLH and incubated with 5μg/ml of 1E4. The values represent the average absorbance at 490 nm of duplicate wells. Panel B, competitive inhibition ELISA analysis of the ability of synthetic peptide m1E41920 to inhibit 1E4 binding with PI antigen. A 96-well microtiter plate was coated with inactivated PI antigen and incubated with 5μg/ml of 1E4 mixed with different concentrations of m1E41920 or control peptide. Points plotted represent the average absorbance at 490 nm of duplicate wells.