Skip to main content
. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Dev Biol. 2013 Jun 17;381(1):10.1016/j.ydbio.2013.06.010. doi: 10.1016/j.ydbio.2013.06.010

Figure 8. Fibronectin and integrin α5 regulate Fgf8 signaling in vivo and in vitro.

Figure 8

mRNA expression of downstream signaling reporters of Fgf8 signaling in E8.75 embryos. A–B. Control (top) and FN-null mutants (bottom). C–D. Control (top) and integrin α5-null mutants (bottom). E. Cell adhesion to FN but not to poly-d-Lys potentiates Fgf8 activation of Erk (double arrows). F. Cell adhesion to FN mediates Fgf8 signaling in a dose-dependent manner and does not lead to increases in Erk protein levels (bottom gel). Activation of Erk in response to cell adhesion to FN and increased doses of Fgf8 is quantified in G.