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. 2013 Nov 19;11(11):e1001715. doi: 10.1371/journal.pbio.1001715

Figure 2. All three GAL cluster genes are rapidly induced from repressed conditions upon loss of DBP2 or the RNA decay factors XRN1 and DCP2.

Figure 2

(A–C) GAL7 (A), GAL10 (B), and GAL1 (C) are rapidly induced from repressed conditions in dbp2Δ, xrn1Δ, and dcp2Δ strains. Induction assays were conducted as in Figure 1 with isogenic xrn1Δ, dbp2Δ, dcp2Δ, and wild-type strains. Asterisks mark the GAL10 lncRNA transcripts, which are visible in the xrn1Δ and dcp2Δ strains due to high abundance and the use of dsDNA probes (most visible from 0–90 min). Lag times represent the average of three biological replicates and the s.d. as in Figure 1. (D–F) Induction of GAL7 (D), GAL10 (E), and GAL1 (F) from derepressed (+raffinose) conditions in dbp2Δ, xrn1Δ, and dcp2Δ cells occurs with wild-type kinetics. Transcriptional induction was measured as above following a nutritional shift from derepressed (+raffinose) to activated (+galactose) conditions.