Figure 5. The GAL lncRNAs do not alter the GAL7 or GAL10 transcription profile in xrn1Δ cells when induced from derepressed conditions.

(A–B) The xrn1Δ and xrn1Δ lncRNAΔ strains display superimposable transcriptional induction profiles of GAL7 (A) and GAL10 (B) from derepressed conditions. Isogenic wild-type (closed black circle), lncRNAΔ (closed red square), xrn1Δ (open blue square), and xrn1Δ lncRNAΔ (open green triangle) strains were analyzed for both rapid induction from derepressive conditions (+raffinose) and final, steady-state transcript levels by conducting time courses as above up to 300 min. Resulting induction profiles were plotted as in Figure 3 following normalization to a fully induced GAL “control” and to SCR1. Representative northern blots are shown in Figure S1. (C–D) GAL7 (C) and GAL10 (D) transcriptional induction kinetic profiles are similar between xrn1Δ and xrn1Δ lncRNAΔ cells. The lag times were calculated as above for each individual biological replicate following normalization to SCR1 and are reported as the average with s.d. The T_max and T_1/2 correspond to the time point when transcript levels plateau and the half-time to T_max, respectively. Initial velocities were calculated as the slope of the straight line from the lag time to T_max, with increases most likely reflecting greater transcript production in a given cell population over time. All kinetic parameters were calculated independently for each biological replicate after graphical analysis, after normalization to SCR1 and the control RNA, and are the average of the three replicates with the s.d.