Table 2. Primers used for real-time PCR gene expression analysis.
| Gene1 | Sequences of primers (5′ to 3′) | Accession number2 | Amplicon region | Median Ct | Efficiency3 |
| AGPAT1 | Forward, GGAGTCATCTTCATTGACCGGA | NM_177518.1 | 737–847 | 29.3 | 99% |
| Reverse, GCCCTCAGGAAAAACCCA | |||||
| ApoB | Forward, TCCTTGATTCCACATGCAGCT | XM_003582812.1 | 11567–11666 | 20.6 | 94% |
| Reverse, GGTGTGCAAAGGATGCGTTAG | |||||
| CPT1a | Forward, CTTCCCATTCCGCACTTTC | XM_002699420.2 | 1950–2033 | 22.6 | 93% |
| Reverse, CCATGTCCTTGTAATGAGCCA | |||||
| PC | Forward, CTTCAAGGACTTCACTGCCACC | NM_177946.4 | 3160–3276 | 21.4 | 98% |
| Reverse, GCCAAGGCTTTGATGTGCA | |||||
| PCK1 | Forward, CGAGAGCAAAGAGATACGGTGC | NM_174737.2 | 427–542 | 18.5 | 98% |
| Reverse, TGACATACATGGTGCGACCCT | |||||
| RPS9 | Forward, GAACAAACGTGAGGTCTGGAGG | NM_001101152.2 | 170–261 | 19.2 | 92% |
| Reverse, TTACCTTCGAACAGACGCCG | |||||
| TNFα | Forward, AAGTAACAAGCCGGTAGCCCA | NM_173966.3 | 448–538 | 27.9 | 103% |
| Reverse, CTTCCAGCTTCACACCGTTG |
1
AGPAT1: 1-acylglycerol-3-phosphate O-acyltransferase 1; ApoB: apolipoprotein B; CPT1a: mitochondrial carnitine palmitoyltransferase 1A; PC: pyruvate carboxylase; PCK1: phosphoenolpyruvate carboxykinase 1; RPS9: ribosomal protein S 9; TNFα: tumor necrosis factor α.
2
From NCBI Entrez Nucleotide Database (http://www.ncbi.nlm.nih.gov/sites/entrez?db=nucleotide).
3
The coefficients of determination (R2) for all genes were greater than 0.98.