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. 2009 Jan;296(1):G55–G65. doi: 10.1152/ajpgi.90298.2008

Fig. 1.

Fig. 1.

Analysis of specificity of anti-ferroportin 1 (FPN1) and hephaestin (Heph) antisera. HEK293 cells were transfected with pDsRed, pDsRed-FPN1, pEmGFP, or pEmGFP-Heph and cell lysates were subjected to SDS-PAGE under reducing conditions with subsequent Western blot analysis as described in materials and methods. A: lysates from HEK293 cells transfected with pDsRed (lanes 1 and 3) and pDsRed-FPN1 (lanes 2 and 4) were separated by SDS-PAGE and blotted with anti-DsRed (lanes 1 and 2) or anti-FPN1 (lanes 3 and 4). B: lysates from HEK cells transfected with pEmGFP (lanes 5 and 7) or pEmGFP-Heph (lanes 6 and 8) were separated by SDS-PAGE and blotted with anti-EmGFP (lanes 5 and 6) or anti-Heph (lanes 7 and 8).