Fig. 6.

Ultracentrifugation velocity sedimentation profiles of FPN1, Heph, divalent metal transporter (DMT1), and sodium/hydrogen exchanger 1 (NHE1) prior to and after iron ingestion. The postnuclear fractions of the rat duodenal epithelium at 0 and 1 h after iron ingestion were loaded onto a sucrose gradient and centrifuged at 40,000 g for 60 min as described in materials and methods. Fractions were collected from the bottom to the top of the gradient and subjected to SDS-PAGE and Western blot with antibodies for FPN1, Heph, DMT1, or NHE1.