Table 2. Specific primer sequences for semi-quantitative and real-time PCR.
Primer name | Sequence (5′-3′) | Product (bp) | Application |
RARβ2-RARE-F | CCTCTCTGGCTGTCTGCTTTTG | 190 | ChIP |
RARβ2-RARE-R | CACTTCCTACTACTTCTGTCAC | ChIP | |
GAPDH-promoter-F | TACTAGCGGTTTTACGGGCG | 166 | ChIP |
GAPDH-promoter-R | TCGAACAGGAGGAGCAGAGAGCGA | ChIP | |
RARβ2-F | TGGGTAAATACACCACGAA | 119 | Q-PCR |
RARβ2-R | TTTAGCAAACTCCACGAT | Q-PCR | |
E-cadherin-F | CTACAATGCCGCCATCGCTTACA | 131 | Q-PCR |
E-cadherin-R | GGAAACTCTCTCGGTCCAGCCCA | Q-PCR | |
Involucrin-F | TCCAGTCAATACCCATCAGG | 156 | Q-PCR |
Involucrin-R | TGCTCACATTCTTGCTCAGG | Q-PCR | |
Loricrin-F | AGTGGACTGCGTGAAGAC | 112 | Q-PCR |
Loricrin-R | GCCAGAACCGCTGCTACC | Q-PCR | |
GAPDH-F | CTTAGCACCCCTGGCCAAG | 151 | Q-PCR |
GAPDH-R | GATGTTCTGGAGAGCCCCG | Q-PCR |