Cryomicrotome images using the autofluorescent excitation and emission filters. Left: Transverse section through lung at the level of the heart (H). In this image, the airway walls are white and the pulmonary vasculature is black as the blood does not autofluoresce. Right: Coronal slice reconstructed from serial transverse sections. The red horizontal line indicates the level of the transverse image on the left. Note the fidelity in the reconstructed image and the ability to identify airways. The left and right lungs and lobes are denoted (LL = left lung, Cr = cranial lobe, M = medial lobe, Ca = caudal lobe, and A = accessory lobe).