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. Author manuscript; available in PMC: 2014 Apr 5.
Published in final edited form as: J Proteome Res. 2013 Mar 12;12(4):1996–2004. doi: 10.1021/pr400100k

Figure 3.

Figure 3

Western blot analysis validating proteins found to be significantly changing in response to metabolic acidosis (p-values < 0.05) by either spectral counting (SpC), average total ion current (MS2 TIC) or both. A: The control (ctrl) and acidotic samples protein bands were imaged and quantified and each blot was normalized to a loading control. B: Fold changes from SpC, MS2 TIC and western blotting (WB). The reported data are the mean ± the S.E. of triplicate biological samples. The * indicates error not shown for CA5B MS2 TIC, ± 4.8.