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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.005. doi: 10.1016/j.bbamcr.2013.07.005

Figure 2. FBXL19 regulates RhoA stability.

Figure 2

A. MLE12 cells were transfected with three distinct FBXL19 shRNA plasmids (#1 - #3) for 72 h. RhoA, FBXL19, and β-actin expression were analyzed by immunoblotting. B. MLE12 cells were co-transfected with FBXL19-V5 plasmid and RhoA-V5 or RhoAV14-V5, or RhoAN19-V5 plasmids. Cell lysates were analyzed for RhoA-V5 or RhoAV14-V5, RhoAN19-V5, FBXL19-V5, and β-actin by immunoblotting with V5 tag and β-actin antibodies. C. RhoAN19-V5 over-expressed MLE12 cells were co-transfected with vector alone, shFBXL19, or shFBXL19 + FBXL19-V5 plasmids and then cells were treated with CHX (20 μg/ml) for 0 - 4 h. Cell lysates were analyzed for RhoAN19-V5, FBXL19-V5, FBXL19, and β-actin by immunoblotting with V5 tag, FBXL19, and β-actin antibodies. Shown are representative blots from three independent experiments.