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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.005. doi: 10.1016/j.bbamcr.2013.07.005

Figure 3. FBXL19 interacts with RhoA.

Figure 3

A. MLE12 cells were co-transfected with RhoAN19-V5 and FBXL19-HA plasmids. Cell lysates were subjected to immunoprecipitation with HA tag, followed by V5 tag immunoblotting. Input lysates were analyzed by immunoblotting with V5 tag and HA tag antibodies. Shown are representative blots from three independent experiments. B. MLE12 cell lysates were subjected to immunoprecipitation with FBXL19 antibody, followed by RhoA immunoblotting. Input lysates were analyzed by immunoblotting with RhoA and β-actin antibodies. Shown are representative blots from three independent experiments. C. MLE12 cells grown on glass-bottom dishes were transfected with FBXL19-GFP plasmid (2 μg) for 48 h followed by MG-132 (20 μM, 18 h) treatment. Localization of FBXL19-GFP (green), RhoA (red), and nuclei (blue) were examined by immunofluorescence staining. Arrows show co-localization of FBXL19 and RhoA. Shown are representative images from three independent experiments. Scale bars represent 10 μm.