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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.005. doi: 10.1016/j.bbamcr.2013.07.005

Figure 5. Erk2 regulates RhoA phosphorylation.

Figure 5

A. MLE12 cells were transfected with Erk2-V5 plasmid for 48 h. Cell lysates were subjected to immunoprecipitation with a RhoA antibody. The precipitates were probed with phospho-serine, phospho-threonine, and RhoA antibodies. Input cell lysates were subjected to V5 tag and β-actin immunoblotting. B. MLE12 cells were transfected with shCont or shErk2 plasmid for 72 h. Cell lysates were subjected to immunoprecipitation with a RhoA antibody. The precipitates were probed with phospho-serine and RhoA antibodies. Input cell lysates were subjected to Erk2 and β-actin immunoblotting. Shown are representative blots from three independent experiments.