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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.005. doi: 10.1016/j.bbamcr.2013.07.005

Figure 7. FBXL19 regulates cell proliferation.

Figure 7

A. MLE12 cells were transfected with shCont and shFBXL19 plasmid for 72 h. Cell proliferation were measured. *p<0.01, compared to shCont. B. MLE12 cells were transfected with FBXL19-V5 plasmid and then cells were treated with 2 % of FBS for 30 min. Cell lysates were analyzed for FBXL19-V5, phospho-p27, and β-actin by immunoblotting with V5 tag, p-p27, and β-actin antibodies. C. MLE12 cells were co-transfected with RhoA-V5 and FBXL19-HA. Cell numbers were accounted for 0 – 3 days. D. MLE12 cells were transfected with shCont, shRhoA, RhoA-V5, or RhoAK135R plasmids as indicted. Cells were treated with or without 2% of FBS for 48 h and cell proliferation were measured. Cell proliferation was normalized to the cells without FBS (veh). *p<0.01, compared to untransfected cells; **p<0.05, compared to shRhoA transfected cells.