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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Biochim Biophys Acta. 2013 Jul 18;1833(12):10.1016/j.bbamcr.2013.07.005. doi: 10.1016/j.bbamcr.2013.07.005

Figure 8. FBXL19 regulates stress fiber formation.

Figure 8

A. MLE12 cells were transfected with FBXL19-V5 plasmid and then cells were treated with LPA (5 μM) for 5 min. Cell lysates were analyzed for phospho-MLC, FBXL19-V5, and β-actin by immunoblotting with p-MLC, V5 tag, and β-actin antibodies. B. MLE12 cells were transfected with empty vector (a and b) or FBXL19-V5 plasmid (c-h) and then cells were treated with LPA (5 μM, 10 min) (b, f-h). Cells were fixed and actin filaments were stained with phalloidin (red), FBXL19-V5 was stained with a V5 tag antibody (green), and nuclei were stained with DAPI (blue). Shown are representative images from three independent experiments. Scale bar represents 2 μm.