Effect of PYY3–36 treatment on p-ERK1/2 activation in the PVN. A, E, and I show low-magnification images of p-ERK1/2 and AVP (shown in pseudocolored red hue for better viewing) colocalization. B, F, J and C, G, K are magnified images (individual channels) of the areas outlined by the dotted rectangles in the leftmost column. D, H, and L are images from the combined channels. Open arrowheads indicate neurons positive for both p-ERK1/2 and AVP; filled arrowheads designate neurons positive for p-ERK1/2 only.