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The effect of Bcl-xL and/or Mcl-1 knock-down on gemcitabine-induced apoptosis in PaCa.
Cells were transfected with Bcl-xL and/or Mcl-1 siRNA for 24h, after which cells were treated with 1 μg/ml gemcitabine for further 72h. DNA fragmentation and the cleavage of PARP were quantified by Cell Death ELISA (A) and Western blot (B), respectively. Results are representative of three independent experiments and are presented as mean ± SD (bars). *p<0.05.
