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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Methods. 2013 Jun 29;64(2):10.1016/j.ymeth.2013.06.019. doi: 10.1016/j.ymeth.2013.06.019

Fig. 1.

Fig. 1

Device configuration and experimental workflow: (A) A NanoVelcro CTC Chip is composed of an overlaid PDMS-based chaotic mixer, a patterned silicon nanowire (SiNW) substrate, and a multilayer chip holder to assemble both functional components together. (B) Silanation reaction and NHS chemistry were employed to covalently link streptavidin onto the SiNW substrate, allowing conjugation of biotinylated anti-EpCAM prior to CTC detection studies. (C) The workflow enables CTC capture and enumeration in 6 hours.