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. 2013 Nov 19;8(11):e79622. doi: 10.1371/journal.pone.0079622

Figure 4. Localization of GFP and GUS in vegetative and reproductive tissues of GFP::P85–P87::GUS transgenic Arabidopsis plants.

Figure 4

(A–E) Confocal laser scanning microscopic analysis of GFP expression under At4g35985 promoter (P85) in three-weeks-old Arabidopsis seedlings. GFP expression in leaf midrib (A), leaf trichomes (B), apical meristematic region (C), primary root (D) and lateral root (E). Green fluorescence image of GFP (left); bright field image (middle), superimposed image (right) are shown. Bar represents 250 µm in each image. (F–K) Histochemical GUS localization data for the At4g35987 promoter (P87) in vegetative tissues of Arabidopsis plants. (F) GUS expression in the apical meristematic region (indicated by arrow), leaf tip, and root parts in 5-day-old Arabidopsis seedlings, bar 1 mm; (G) GUS expression in leaf tip and very young bud (orange arrow) of two-week-old Arabidopsis seedlings, bar represents 1 mm; (H) GUS expression at the junction of young bud and stem, bar represents 1 mm; (I) GUS expression in leaf tip (black arrow) and hydathode (orange arrow) at aerial leaves of three-week-old Arabidopsis plants; bar represents 1 mm; (J) GUS expression in root tip of five-day-old seedlings, bar represents 100 µm; and (K) GUS expression in root stele and emerging lateral root of five-day-old seedling, bar represents 100 µm, are presented. (L–N) Confocal laser scanning microscopic analysis of GFP expression under the At4g35985 promoter (P85) in reproductive tissues of Arabidopsis plants. GFP expression in anther (white arrow), stigma (orange arrow) in flower (L); tip of immature silique (M); and abscission zone (white arrow) in the base of silique (N) are presented. Green fluorescence image of GFP (left); bright field image (middle), superimposed image (right) are shown. Bar 250 µm in each image. (O–S) Histochemical GUS detection for At4g35987 promoter (P87) in reproductive tissues of Arabidopsis plants. (O) GUS expression in emerging floral bud (indicated by white arrow), bar represents 1 mm; (P) GUS expression in developing floral bud, bar represents 1 mm; (Q) GUS expression in stigma (white arrow) and anther (orange arrow) of Arabidopsis flower, bar represents 1 mm; (R) GUS expression in filament (black arrow) and pollen grains in anther (orange arrow), bar represents 200 µm; (S) GUS expression in abscission zone (white arrow) and immature seeds (orange arrow) of silique, bar represents 1 mm.