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. 2013 Nov 19;8(11):e79622. doi: 10.1371/journal.pone.0079622

Figure 5. Localization of GFP and GUS in vegetative and reproductive tissues of GFP::P87–P85::GUS transgenic Arabidopsis plants.

Figure 5

(A–D) Confocal laser scanning microscopic analysis of GFP expression under the At4g35987 promoter (P87) in Arabidopsis plants. GFP expression in peripheral border region of cotyledon in five-days-old Arabidopsis seedling (A); hydathode in three-weeks-old seedling (B), apical meristematic region in three-week-old seedlings (C); and primary root tip in five-day-old seedling (D). Green fluorescence image of GFP (left); bright field image (middle), superimposed image (right) are depicted. Bar represents 250 µm in each image. (E–J) Histological GUS localization data for the At4g35985 promoter (P85) in vegetative tissues of Arabidopsis plants. (E) GUS expression in the midrib of cotyledons, young stem and root in five-day-old Arabidopsis seedling, bar represents 1 mm; (F) moderate GUS expression in leaf tip, hydathode and strong GUS expression in leaf venation at aerial leaf in three-week-old Arabidopsis plants, arrow indicates expression at leaf tip and hydathode, bar represents 1 mm; (G) strong GUS expression in root tip of five-day-old seedlings, bar represents 100 µm; (H) strong GUS expression in root stele and emerging lateral root of five-day-old seedlings, bar represents 100 µm; (I) GUS staining of leaf trichomes. bar represents 200 µm; (J) GUS staining of trichomes in young bud, bar represents 200 µm. (K–M) Confocal laser scanning microscopic analysis of GFP expression under the At4g35987 promoter (P87) in reproductive tissues of Arabidopsis plants. GFP expression in anther (white arrow) and stigma (orange arrow) in flower (K); tip of immature silique (L); and abscission zone (white arrow) in the base of silique (M) are depicted. Green fluorescence image of GFP (left); bright field image (middle), superimposed image (right) are shown. Bar represents 250 µm in each image. (N–S) Histochemical GUS localization under the At4g35985 promoter (P85) in reproductive tissues of Arabidopsis plants. (N) GUS expression in floral bud, bar represents 1 mm; (O) GUS expression in anther (orange arrow) and stigma (white arrow) in Arabidopsis flower, bar represents 1 mm; (P) GUS expression in anther (orange arrow) and petals (black arrow), bar represents 200 µm; (Q) GUS expression in pollen grains bearing anther, pollen grains are indicated by orange arrow. bar represents 200 µm; (R) GUS expression in developing silique. bar represents 1 mm; (S) GUS expression in developed silique before senescence, bar represents 1 mm.